Primers Available
Organism: Testudo marginata mitochondrion
Repeat:(GCA)4
Start:8744 End:8755
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ATCCAAGCCCATGACCACTG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ATGGTGTCCTTGGAAGGTTC | |||||||||||||
| Tm_F_1 | 60.034 | Size_F_1 | 20 | Tm_R_1 | 57.413 | Size_R_1 | 20 | PRODUCT Size | 179 bp | Primer Start | 8717 | Primer End | 8895 |
| FORWARD_PRIMER_2 | |||||||||||||
| AAGCCCATGACCACTGACAG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ATGGTGTCCTTGGAAGGTTC | |||||||||||||
| Tm_F_2 | 59.963 | Size_F_2 | 20 | Tm_R_2 | 57.413 | Size_R_2 | 20 | PRODUCT Size | 175 bp | Primer Start | 8721 | Primer End | 8895 |
| FORWARD_PRIMER_3 | |||||||||||||
| ATCCAAGCCCATGACCACTG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AGTATGGTGTCCTTGGAAGGT | |||||||||||||
| Tm_F_3 | 60.034 | Size_F_3 | 20 | Tm_R_3 | 58.367 | Size_R_3 | 21 | PRODUCT Size | 182 bp | Primer Start | 8717 | Primer End | 8898 |