Primers Available
Organism: Nicotiana tomentosiformis chloroplast
Repeat:(TTA)4
Start:53712 End:53723
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CGAACCGTAGACCTTCTCGG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AGCACATGGAGCCATTTCCT | |||||||||||||
| Tm_F_1 | 59.902 | Size_F_1 | 20 | Tm_R_1 | 59.666 | Size_R_1 | 20 | PRODUCT Size | 185 bp | Primer Start | 53676 | Primer End | 53860 |
| FORWARD_PRIMER_2 | |||||||||||||
| CGAACCGTAGACCTTCTCGG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GCCAGAGACAGACCTACGTC | |||||||||||||
| Tm_F_2 | 59.902 | Size_F_2 | 20 | Tm_R_2 | 59.547 | Size_R_2 | 20 | PRODUCT Size | 258 bp | Primer Start | 53676 | Primer End | 53933 |
| FORWARD_PRIMER_3 | |||||||||||||
| GGGCTATACGGACTCGAACC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AGCACATGGAGCCATTTCCT | |||||||||||||
| Tm_F_3 | 59.687 | Size_F_3 | 20 | Tm_R_3 | 59.666 | Size_R_3 | 20 | PRODUCT Size | 199 bp | Primer Start | 53662 | Primer End | 53860 |