Primers Available
Organism: Nicotiana tomentosiformis chloroplast
Repeat:(T)14
Start:132573 End:132586
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CTTTCGTCTCCCCAGCCATT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GCTCGACGCCAGGATGATAA | |||||||||||||
| Tm_F_1 | 60.035 | Size_F_1 | 20 | Tm_R_1 | 59.968 | Size_R_1 | 20 | PRODUCT Size | 222 bp | Primer Start | 132430 | Primer End | 132651 |
| FORWARD_PRIMER_2 | |||||||||||||
| CTTTCGTCTCCCCAGCCATT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TACTGTAGGGGAGGTCCTGC | |||||||||||||
| Tm_F_2 | 60.035 | Size_F_2 | 20 | Tm_R_2 | 60.032 | Size_R_2 | 20 | PRODUCT Size | 251 bp | Primer Start | 132430 | Primer End | 132680 |
| FORWARD_PRIMER_3 | |||||||||||||
| TTCGTCTCCCCAGCCATTTC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GCTCGACGCCAGGATGATAA | |||||||||||||
| Tm_F_3 | 60.035 | Size_F_3 | 20 | Tm_R_3 | 59.968 | Size_R_3 | 20 | PRODUCT Size | 220 bp | Primer Start | 132432 | Primer End | 132651 |