Primers Available
Organism: Nicotiana sylvestris chloroplast
Repeat:(TTTA)3
Start:31111 End:31122
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCCATACCGCCCCAAACTTG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CACTGGAAGTGGAAGAGCTAT | |||||||||||||
| Tm_F_1 | 60.323 | Size_F_1 | 20 | Tm_R_1 | 57.097 | Size_R_1 | 21 | PRODUCT Size | 165 bp | Primer Start | 31065 | Primer End | 31229 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCCATACCGCCCCAAACTTG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TGGGTTTGTAACTATGTGACCACT | |||||||||||||
| Tm_F_2 | 60.323 | Size_F_2 | 20 | Tm_R_2 | 59.897 | Size_R_2 | 24 | PRODUCT Size | 185 bp | Primer Start | 31065 | Primer End | 31249 |
| FORWARD_PRIMER_3 | |||||||||||||
| ATCCATACCGCCCCAAACTT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CACTGGAAGTGGAAGAGCTAT | |||||||||||||
| Tm_F_3 | 59.371 | Size_F_3 | 20 | Tm_R_3 | 57.097 | Size_R_3 | 21 | PRODUCT Size | 166 bp | Primer Start | 31064 | Primer End | 31229 |