Primers Available
Organism: Nicotiana sylvestris chloroplast
Repeat:(TTA)4
Start:53809 End:53820
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CGAACCGTAGACCTTCTCGG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AGCACATGGAGCCATTTCCT | |||||||||||||
| Tm_F_1 | 59.902 | Size_F_1 | 20 | Tm_R_1 | 59.666 | Size_R_1 | 20 | PRODUCT Size | 184 bp | Primer Start | 53773 | Primer End | 53956 |
| FORWARD_PRIMER_2 | |||||||||||||
| CGAACCGTAGACCTTCTCGG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GCCAGAGACAGACCTACGTC | |||||||||||||
| Tm_F_2 | 59.902 | Size_F_2 | 20 | Tm_R_2 | 59.547 | Size_R_2 | 20 | PRODUCT Size | 257 bp | Primer Start | 53773 | Primer End | 54029 |
| FORWARD_PRIMER_3 | |||||||||||||
| GGGCTATACGGACTCGAACC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AGCACATGGAGCCATTTCCT | |||||||||||||
| Tm_F_3 | 59.687 | Size_F_3 | 20 | Tm_R_3 | 59.666 | Size_R_3 | 20 | PRODUCT Size | 198 bp | Primer Start | 53759 | Primer End | 53956 |