Primers Available
Organism: Nicotiana sylvestris chloroplast
Repeat:(TAAA)3
Start:426 End:437
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TGCAAGAAAATAACCTCTCCTTCT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ACCGTGCTAACCTTGGTATGG | |||||||||||||
| Tm_F_1 | 58.119 | Size_F_1 | 24 | Tm_R_1 | 60.066 | Size_R_1 | 21 | PRODUCT Size | 277 bp | Primer Start | 356 | Primer End | 632 |
| FORWARD_PRIMER_2 | |||||||||||||
| TGCAAGAAAATAACCTCTCCTTCT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CCGTGCTAACCTTGGTATGGA | |||||||||||||
| Tm_F_2 | 58.119 | Size_F_2 | 24 | Tm_R_2 | 59.791 | Size_R_2 | 21 | PRODUCT Size | 276 bp | Primer Start | 356 | Primer End | 631 |
| FORWARD_PRIMER_3 | |||||||||||||
| TGCAAGAAAATAACCTCTCCTTCT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TCCCTCTAGACCTAGCTGCT | |||||||||||||
| Tm_F_3 | 58.119 | Size_F_3 | 24 | Tm_R_3 | 58.773 | Size_R_3 | 20 | PRODUCT Size | 226 bp | Primer Start | 356 | Primer End | 581 |