Primers Available
Organism: Nicotiana sylvestris chloroplast
Repeat:(T)13
Start:132843 End:132855
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GAACCTTTCCTCTCCCCAGC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GCTCGACGCCAGGATGATAA | |||||||||||||
| Tm_F_1 | 60.035 | Size_F_1 | 20 | Tm_R_1 | 59.968 | Size_R_1 | 20 | PRODUCT Size | 225 bp | Primer Start | 132696 | Primer End | 132920 |
| FORWARD_PRIMER_2 | |||||||||||||
| GAACCTTTCCTCTCCCCAGC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TACTGTAGGGGAGGTCCTGC | |||||||||||||
| Tm_F_2 | 60.035 | Size_F_2 | 20 | Tm_R_2 | 60.032 | Size_R_2 | 20 | PRODUCT Size | 254 bp | Primer Start | 132696 | Primer End | 132949 |
| FORWARD_PRIMER_3 | |||||||||||||
| GAACCTTTCCTCTCCCCAGC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AATAGCTCGACGCCAGGATG | |||||||||||||
| Tm_F_3 | 60.035 | Size_F_3 | 20 | Tm_R_3 | 59.967 | Size_R_3 | 20 | PRODUCT Size | 229 bp | Primer Start | 132696 | Primer End | 132924 |