Primers Available
Organism: Nicotiana sylvestris chloroplast
Repeat:(T)13
Start:12875 End:12887
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCATTTGGCTCTCACGCTCA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TGCTGAATCGACGACCTATTCT | |||||||||||||
| Tm_F_1 | 59.679 | Size_F_1 | 20 | Tm_R_1 | 59.312 | Size_R_1 | 22 | PRODUCT Size | 244 bp | Primer Start | 12690 | Primer End | 12933 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCATTTGGCTCTCACGCTCA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TCTAATGCTGAATCGACGACCT | |||||||||||||
| Tm_F_2 | 59.679 | Size_F_2 | 20 | Tm_R_2 | 59.312 | Size_R_2 | 22 | PRODUCT Size | 249 bp | Primer Start | 12690 | Primer End | 12938 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCGGAGGACTCTTCTGACAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AGACGCAGCATGGTACTCTT | |||||||||||||
| Tm_F_3 | 57.712 | Size_F_3 | 20 | Tm_R_3 | 59.102 | Size_R_3 | 20 | PRODUCT Size | 266 bp | Primer Start | 12819 | Primer End | 13084 |