Primers Available
Organism: Nicotiana sylvestris chloroplast
Repeat:(A)14
Start:46118 End:46131
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCACGTGTTTTTCTCCTCGT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GCTACCTATGCCTAGATCACGG | |||||||||||||
| Tm_F_1 | 57.41 | Size_F_1 | 20 | Tm_R_1 | 59.835 | Size_R_1 | 22 | PRODUCT Size | 209 bp | Primer Start | 46067 | Primer End | 46275 |
| FORWARD_PRIMER_2 | |||||||||||||
| ACAGAACCTCTAGGCGTAACC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ACCTATTACAGAGATGGTGCGA | |||||||||||||
| Tm_F_2 | 59.176 | Size_F_2 | 21 | Tm_R_2 | 58.703 | Size_R_2 | 22 | PRODUCT Size | 252 bp | Primer Start | 45909 | Primer End | 46160 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCACGTGTTTTTCTCCTCGT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ACCTATGCCTAGATCACGGA | |||||||||||||
| Tm_F_3 | 57.41 | Size_F_3 | 20 | Tm_R_3 | 57.322 | Size_R_3 | 20 | PRODUCT Size | 206 bp | Primer Start | 46067 | Primer End | 46272 |