Primers Available
Organism: Nicotiana sylvestris chloroplast
Repeat:(A)13
Start:57350 End:57362
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CCACTGTCAAGGGGGAAGTT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ACAAGGTCTACTCGACACGA | |||||||||||||
| Tm_F_1 | 59.521 | Size_F_1 | 20 | Tm_R_1 | 58.112 | Size_R_1 | 20 | PRODUCT Size | 260 bp | Primer Start | 57295 | Primer End | 57554 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCACTGTCAAGGGGGAAGTT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CAACAAGGTCTACTCGACACGA | |||||||||||||
| Tm_F_2 | 59.521 | Size_F_2 | 20 | Tm_R_2 | 60.031 | Size_R_2 | 22 | PRODUCT Size | 262 bp | Primer Start | 57295 | Primer End | 57556 |
| FORWARD_PRIMER_3 | |||||||||||||
| CCACTGTCAAGGGGGAAGTT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ACAAGGTCTACTCGACACGAA | |||||||||||||
| Tm_F_3 | 59.521 | Size_F_3 | 20 | Tm_R_3 | 58.775 | Size_R_3 | 21 | PRODUCT Size | 260 bp | Primer Start | 57295 | Primer End | 57554 |