Primers Available
Organism: Acorus calamus plastid
Repeat:(ATTCAG)4
Start:147747 End:147770
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCCAATCTAATTGTTCCAGAGCA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CGCATCTTCTCTTTGTTTCCCG | |||||||||||||
| Tm_F_1 | 57.822 | Size_F_1 | 23 | Tm_R_1 | 60.16 | Size_R_1 | 22 | PRODUCT Size | 222 bp | Primer Start | 147683 | Primer End | 147904 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCCAATCTAATTGTTCCAGAGCA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TGTTGCTGGATATCTCGTTCGT | |||||||||||||
| Tm_F_2 | 57.822 | Size_F_2 | 23 | Tm_R_2 | 59.834 | Size_R_2 | 22 | PRODUCT Size | 245 bp | Primer Start | 147683 | Primer End | 147927 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCCAATCTAATTGTTCCAGAGCA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CGTTCGTACGCATCTTCTCT | |||||||||||||
| Tm_F_3 | 57.822 | Size_F_3 | 23 | Tm_R_3 | 57.824 | Size_R_3 | 20 | PRODUCT Size | 230 bp | Primer Start | 147683 | Primer End | 147912 |