Primers Available
Organism: Acorus calamus plastid
Repeat:(A)15
Start:7892 End:7906
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CATTCCGGGGAGCAAAGACT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ACGAAGAGTTTGAGAGTAAGCA | |||||||||||||
| Tm_F_1 | 60.035 | Size_F_1 | 20 | Tm_R_1 | 57.414 | Size_R_1 | 22 | PRODUCT Size | 275 bp | Primer Start | 7678 | Primer End | 7952 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCCGGGGAGCAAAGACTCTA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ACGAAGAGTTTGAGAGTAAGCA | |||||||||||||
| Tm_F_2 | 59.959 | Size_F_2 | 20 | Tm_R_2 | 57.414 | Size_R_2 | 22 | PRODUCT Size | 272 bp | Primer Start | 7681 | Primer End | 7952 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCTATTCCCTCCCCCTCTTTT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TCCTCACGTCCAGGATTACG | |||||||||||||
| Tm_F_3 | 57.474 | Size_F_3 | 21 | Tm_R_3 | 58.899 | Size_R_3 | 20 | PRODUCT Size | 169 bp | Primer Start | 7868 | Primer End | 8036 |