Primers Available
Organism: Cobitis sinensis mitochondrion
Repeat:(TA)6
Start:16444 End:16455
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CCTTTTTGGTTTCTGCGCGA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TCCTGTATGTTAGGGTTGACAGA | |||||||||||||
| Tm_F_1 | 59.97 | Size_F_1 | 20 | Tm_R_1 | 58.578 | Size_R_1 | 23 | PRODUCT Size | 250 bp | Primer Start | 16296 | Primer End | 16545 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCCCCTTTTTGGTTTCTGCG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TCCTGTATGTTAGGGTTGACAGA | |||||||||||||
| Tm_F_2 | 59.967 | Size_F_2 | 20 | Tm_R_2 | 58.578 | Size_R_2 | 23 | PRODUCT Size | 253 bp | Primer Start | 16293 | Primer End | 16545 |
| FORWARD_PRIMER_3 | |||||||||||||
| ACCCCGAAACCAAGGAAGAC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TCCTGTATGTTAGGGTTGACAGA | |||||||||||||
| Tm_F_3 | 59.89 | Size_F_3 | 20 | Tm_R_3 | 58.578 | Size_R_3 | 23 | PRODUCT Size | 181 bp | Primer Start | 16365 | Primer End | 16545 |