Primers Available
Organism: Cucumis sativus chloroplast
Repeat:(TTTCA)3
Start:147110 End:147124
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TGGCCACATATTTTTCCGGC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TGATTCATCGCCAGAGATGG | |||||||||||||
| Tm_F_1 | 59.179 | Size_F_1 | 20 | Tm_R_1 | 57.461 | Size_R_1 | 20 | PRODUCT Size | 260 bp | Primer Start | 147048 | Primer End | 147307 |
| FORWARD_PRIMER_2 | |||||||||||||
| TGGCCACATATTTTTCCGGC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GTTGATTCATCGCCAGAGATGG | |||||||||||||
| Tm_F_2 | 59.179 | Size_F_2 | 20 | Tm_R_2 | 59.453 | Size_R_2 | 22 | PRODUCT Size | 262 bp | Primer Start | 147048 | Primer End | 147309 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCCGGCTAAAGGAATGGGAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ACGCAAGAAAAGTACGTCGA | |||||||||||||
| Tm_F_3 | 58.709 | Size_F_3 | 20 | Tm_R_3 | 57.876 | Size_R_3 | 20 | PRODUCT Size | 271 bp | Primer Start | 147062 | Primer End | 147332 |