Primers Available
Organism: Cucumis sativus chloroplast
Repeat:(TTATT)3
Start:48311 End:48325
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GTTAGTCCCGGTAAAGCCCC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ACTTTACTGGATTTCGCTTCATTT | |||||||||||||
| Tm_F_1 | 60.108 | Size_F_1 | 20 | Tm_R_1 | 57.425 | Size_R_1 | 24 | PRODUCT Size | 157 bp | Primer Start | 48236 | Primer End | 48392 |
| FORWARD_PRIMER_2 | |||||||||||||
| GATCGTTTCCGGTTTTGGGC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ACTTTACTGGATTTCGCTTCATTT | |||||||||||||
| Tm_F_2 | 60.109 | Size_F_2 | 20 | Tm_R_2 | 57.425 | Size_R_2 | 24 | PRODUCT Size | 186 bp | Primer Start | 48207 | Primer End | 48392 |
| FORWARD_PRIMER_3 | |||||||||||||
| GGCCTTCTGTAACGCGACAT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TCATGTCGTAGGAACTTACTTACCT | |||||||||||||
| Tm_F_3 | 60.74 | Size_F_3 | 20 | Tm_R_3 | 59.052 | Size_R_3 | 25 | PRODUCT Size | 280 bp | Primer Start | 48281 | Primer End | 48560 |