Primers Available
Organism: Cucumis sativus chloroplast
Repeat:(TAA)4
Start:74303 End:74314
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TGGAAGCGTAACAACGGGTT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CCAATACGCAATGGTAGATGGG | |||||||||||||
| Tm_F_1 | 60.179 | Size_F_1 | 20 | Tm_R_1 | 59.186 | Size_R_1 | 22 | PRODUCT Size | 232 bp | Primer Start | 74216 | Primer End | 74447 |
| FORWARD_PRIMER_2 | |||||||||||||
| TGGAAGCGTAACAACGGGTT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ACGCAATGGTAGATGGGAATTG | |||||||||||||
| Tm_F_2 | 60.179 | Size_F_2 | 20 | Tm_R_2 | 59.048 | Size_R_2 | 22 | PRODUCT Size | 227 bp | Primer Start | 74216 | Primer End | 74442 |
| FORWARD_PRIMER_3 | |||||||||||||
| TGGAAGCGTAACAACGGGTT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CGCAATGGTAGATGGGAATTGA | |||||||||||||
| Tm_F_3 | 60.179 | Size_F_3 | 20 | Tm_R_3 | 58.786 | Size_R_3 | 22 | PRODUCT Size | 226 bp | Primer Start | 74216 | Primer End | 74441 |