Primers Available
Organism: Cucumis sativus chloroplast
Repeat:(T)18c(A)12
Start:72961 End:72991
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCTAGCAGACCCAGAGCAGT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AGCCGCTTCAATGGGATCTT | |||||||||||||
| Tm_F_1 | 59.96 | Size_F_1 | 20 | Tm_R_1 | 59.743 | Size_R_1 | 20 | PRODUCT Size | 238 bp | Primer Start | 72836 | Primer End | 73073 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCTAGCAGACCCAGAGCAGT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ACGTCTAGCATTCCCTCACG | |||||||||||||
| Tm_F_2 | 59.96 | Size_F_2 | 20 | Tm_R_2 | 59.544 | Size_R_2 | 20 | PRODUCT Size | 190 bp | Primer Start | 72836 | Primer End | 73025 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCTAGCAGACCCAGAGCAGT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GTCTAGCATTCCCTCACGCT | |||||||||||||
| Tm_F_3 | 59.96 | Size_F_3 | 20 | Tm_R_3 | 59.538 | Size_R_3 | 20 | PRODUCT Size | 188 bp | Primer Start | 72836 | Primer End | 73023 |