Primers Available
Organism: Cucumis sativus chloroplast
Repeat:(T)14
Start:86663 End:86676
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GGTAGATGCTCGGGACCAAG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TGGGGTTATCCTGCACTTGG | |||||||||||||
| Tm_F_1 | 59.895 | Size_F_1 | 20 | Tm_R_1 | 59.669 | Size_R_1 | 20 | PRODUCT Size | 258 bp | Primer Start | 86535 | Primer End | 86792 |
| FORWARD_PRIMER_2 | |||||||||||||
| GGTAGATGCTCGGGACCAAG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CCCTTGGGGTTATCCTGCAC | |||||||||||||
| Tm_F_2 | 59.895 | Size_F_2 | 20 | Tm_R_2 | 60.396 | Size_R_2 | 20 | PRODUCT Size | 262 bp | Primer Start | 86535 | Primer End | 86796 |
| FORWARD_PRIMER_3 | |||||||||||||
| TGATCGCGCCATTTACCTCC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AACCCTGTAGACCATCCCCA | |||||||||||||
| Tm_F_3 | 60.532 | Size_F_3 | 20 | Tm_R_3 | 59.88 | Size_R_3 | 20 | PRODUCT Size | 225 bp | Primer Start | 86640 | Primer End | 86864 |