Primers Available
Organism: Cucumis sativus chloroplast
Repeat:(T)13
Start:100519 End:100531
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ACCGGGACCAATTCAGTCAC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CCGCGGGATAGGGATAGAGA | |||||||||||||
| Tm_F_1 | 59.964 | Size_F_1 | 20 | Tm_R_1 | 60.034 | Size_R_1 | 20 | PRODUCT Size | 266 bp | Primer Start | 100435 | Primer End | 100700 |
| FORWARD_PRIMER_2 | |||||||||||||
| GTGACCGGGACCAATTCAGT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CCGCGGGATAGGGATAGAGA | |||||||||||||
| Tm_F_2 | 59.964 | Size_F_2 | 20 | Tm_R_2 | 60.034 | Size_R_2 | 20 | PRODUCT Size | 269 bp | Primer Start | 100432 | Primer End | 100700 |
| FORWARD_PRIMER_3 | |||||||||||||
| GACCGGGACCAATTCAGTCA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CCGCGGGATAGGGATAGAGA | |||||||||||||
| Tm_F_3 | 59.676 | Size_F_3 | 20 | Tm_R_3 | 60.034 | Size_R_3 | 20 | PRODUCT Size | 267 bp | Primer Start | 100434 | Primer End | 100700 |