Primers Available
Organism: Cucumis sativus chloroplast
Repeat:(AGTCGA)3agcaggaatagaagttgaagtt(GAAGTC)3gaagcaggaatagaagttgaagtcgaagcaggaataaaagttgaagtcgaagtcgaagcaggaatagaagtt(GAAGTC)3gaagcaggaatagaagtcgaagtcgaagcaggaatagaagcagg(AGTAGA)3
Start:129055 End:129264
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TGGTTCGTCCTTTGGTTCGT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TCCTACTTCTACTCCTACTTCTACTCC | |||||||||||||
| Tm_F_1 | 59.821 | Size_F_1 | 20 | Tm_R_1 | 59.929 | Size_R_1 | 27 | PRODUCT Size | 280 bp | Primer Start | 129015 | Primer End | 129294 |
| FORWARD_PRIMER_2 | |||||||||||||
| TGGTTCGTCCTTTGGTTCGT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CCTACTTCTACTCCTACTTCTACTCC | |||||||||||||
| Tm_F_2 | 59.821 | Size_F_2 | 20 | Tm_R_2 | 58.781 | Size_R_2 | 26 | PRODUCT Size | 279 bp | Primer Start | 129015 | Primer End | 129293 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCGTCCTTTGGTTCGTCCTT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ACTCCTACTTCTACTCCTACTTCT | |||||||||||||
| Tm_F_3 | 59.246 | Size_F_3 | 20 | Tm_R_3 | 57.104 | Size_R_3 | 24 | PRODUCT Size | 278 bp | Primer Start | 129019 | Primer End | 129296 |