Primers Available
Organism: Cucumis sativus chloroplast
Repeat:(AAAT)4
Start:152176 End:152191
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TATCCCCTCCTGTTCCGGTC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TTTGTCCCTTCCAGGCGATC | |||||||||||||
| Tm_F_1 | 60.398 | Size_F_1 | 20 | Tm_R_1 | 60.035 | Size_R_1 | 20 | PRODUCT Size | 227 bp | Primer Start | 152143 | Primer End | 152369 |
| FORWARD_PRIMER_2 | |||||||||||||
| GTGTATCCCCTCCTGTTCCG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TTTGTCCCTTCCAGGCGATC | |||||||||||||
| Tm_F_2 | 59.536 | Size_F_2 | 20 | Tm_R_2 | 60.035 | Size_R_2 | 20 | PRODUCT Size | 230 bp | Primer Start | 152140 | Primer End | 152369 |
| FORWARD_PRIMER_3 | |||||||||||||
| CGTGTATCCCCTCCTGTTCC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTTGTCCCTTCCAGGCGATC | |||||||||||||
| Tm_F_3 | 59.536 | Size_F_3 | 20 | Tm_R_3 | 60.035 | Size_R_3 | 20 | PRODUCT Size | 231 bp | Primer Start | 152139 | Primer End | 152369 |