Primers Available
Organism: Cucumis sativus chloroplast
Repeat:(A)16
Start:9254 End:9269
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CAGTACCTAGCCGGGCTTTT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AGGAATCGAACCTTGATTCTATATCA | |||||||||||||
| Tm_F_1 | 59.748 | Size_F_1 | 20 | Tm_R_1 | 57.805 | Size_R_1 | 26 | PRODUCT Size | 176 bp | Primer Start | 9179 | Primer End | 9354 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCTGGCCTGGTCAGTACCTA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AGGAATCGAACCTTGATTCTATATCA | |||||||||||||
| Tm_F_2 | 60.325 | Size_F_2 | 20 | Tm_R_2 | 57.805 | Size_R_2 | 26 | PRODUCT Size | 187 bp | Primer Start | 9168 | Primer End | 9354 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCAGTACCTAGCCGGGCTTT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AGGAATCGAACCTTGATTCTATATCA | |||||||||||||
| Tm_F_3 | 60.617 | Size_F_3 | 20 | Tm_R_3 | 57.805 | Size_R_3 | 26 | PRODUCT Size | 177 bp | Primer Start | 9178 | Primer End | 9354 |