Primers Available
Organism: Cucumis sativus chloroplast
Repeat:(A)13
Start:83396 End:83408
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GGCTCCGCTGTTATCTGCTA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TCCAAAATGCCTATACGAACCCA | |||||||||||||
| Tm_F_1 | 59.611 | Size_F_1 | 20 | Tm_R_1 | 60.054 | Size_R_1 | 23 | PRODUCT Size | 233 bp | Primer Start | 83281 | Primer End | 83513 |
| FORWARD_PRIMER_2 | |||||||||||||
| GGCTCCGCTGTTATCTGCTA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CGAACCCAATTCATTACTTCGGG | |||||||||||||
| Tm_F_2 | 59.611 | Size_F_2 | 20 | Tm_R_2 | 59.935 | Size_R_2 | 23 | PRODUCT Size | 218 bp | Primer Start | 83281 | Primer End | 83498 |
| FORWARD_PRIMER_3 | |||||||||||||
| GGCTCCGCTGTTATCTGCTA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CCAAAATGCCTATACGAACCCA | |||||||||||||
| Tm_F_3 | 59.611 | Size_F_3 | 20 | Tm_R_3 | 58.716 | Size_R_3 | 22 | PRODUCT Size | 232 bp | Primer Start | 83281 | Primer End | 83512 |