Primers Available
Organism: Cucumis sativus chloroplast
Repeat:(A)12
Start:3159 End:3170
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TGTTGTTGTACCCCCAAAAAGG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GCAACGTGACTTCCTATACCCA | |||||||||||||
| Tm_F_1 | 59.233 | Size_F_1 | 22 | Tm_R_1 | 60.094 | Size_R_1 | 22 | PRODUCT Size | 221 bp | Primer Start | 3114 | Primer End | 3334 |
| FORWARD_PRIMER_2 | |||||||||||||
| CAATGACTGCAAACCCTTCTGA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GCAACGTGACTTCCTATACCCA | |||||||||||||
| Tm_F_2 | 59.113 | Size_F_2 | 22 | Tm_R_2 | 60.094 | Size_R_2 | 22 | PRODUCT Size | 263 bp | Primer Start | 3072 | Primer End | 3334 |
| FORWARD_PRIMER_3 | |||||||||||||
| TGACTGCAAACCCTTCTGAT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GCAACGTGACTTCCTATACCCA | |||||||||||||
| Tm_F_3 | 57.043 | Size_F_3 | 20 | Tm_R_3 | 60.094 | Size_R_3 | 22 | PRODUCT Size | 260 bp | Primer Start | 3075 | Primer End | 3334 |