Primers Available
Organism: Cucumis sativus chloroplast
Repeat:(A)12
Start:115117 End:115128
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCCCTTTTCCTTTTTGTAGGCT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TTGGTTTAGTGGGGCGATGG | |||||||||||||
| Tm_F_1 | 57.807 | Size_F_1 | 22 | Tm_R_1 | 60.323 | Size_R_1 | 20 | PRODUCT Size | 189 bp | Primer Start | 115090 | Primer End | 115278 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCCCTTTTCCTTTTTGTAGGCT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GGGGCGATGGGGATTCATAT | |||||||||||||
| Tm_F_2 | 57.807 | Size_F_2 | 22 | Tm_R_2 | 59.374 | Size_R_2 | 20 | PRODUCT Size | 179 bp | Primer Start | 115090 | Primer End | 115268 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCCCTTTTCCTTTTTGTAGGCT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TGGGGCGATGGGGATTCATA | |||||||||||||
| Tm_F_3 | 57.807 | Size_F_3 | 22 | Tm_R_3 | 60.77 | Size_R_3 | 20 | PRODUCT Size | 180 bp | Primer Start | 115090 | Primer End | 115269 |