Primers Available
Organism:Smittium culisetae mitochondrion
Repeat:(TTA)5
Start:18571 End:18585
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TTTTTGAGATATTGGGGATTTATCA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TGCTAATGGTGCAGGATTTTT | |||||||||||||
| Tm_F_1 | 59.939 | Size_F_1 | 25 | Tm_R_1 | 59.6 | Size_R_1 | 21 | PRODUCT Size | 271 bp | Primer Start | 18542 | Primer End | 18812 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTTTGAGATATTGGGGATTTATCA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TGCTAATGGTGCAGGATTTTT | |||||||||||||
| Tm_F_2 | 58.795 | Size_F_2 | 24 | Tm_R_2 | 59.6 | Size_R_2 | 21 | PRODUCT Size | 270 bp | Primer Start | 18543 | Primer End | 18812 |
| FORWARD_PRIMER_3 | |||||||||||||
| TTTTTGAGATATTGGGGATTTATCA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TATGCATGCTAATGGTGCAG | |||||||||||||
| Tm_F_3 | 59.939 | Size_F_3 | 25 | Tm_R_3 | 58.327 | Size_R_3 | 20 | PRODUCT Size | 277 bp | Primer Start | 18542 | Primer End | 18818 |