Primers Available
Organism:Smittium culisetae mitochondrion
Repeat:(CTT)4
Start:22516 End:22527
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CTCTTAATTCACTCATTCTTTTTATCC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TTTTGTAATGAGATGCTAAAGGAA | |||||||||||||
| Tm_F_1 | 57.376 | Size_F_1 | 27 | Tm_R_1 | 57.286 | Size_R_1 | 24 | PRODUCT Size | 100 bp | Primer Start | 22467 | Primer End | 22566 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCTTAATTCACTCATTCTTTTTATCCT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GAGGAATCTGTTGGATTCTATAGTTG | |||||||||||||
| Tm_F_2 | 57.376 | Size_F_2 | 27 | Tm_R_2 | 58.714 | Size_R_2 | 26 | PRODUCT Size | 147 bp | Primer Start | 22468 | Primer End | 22614 |
| FORWARD_PRIMER_3 | |||||||||||||
| CTCTTAATTCACTCATTCTTTTTATCC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GAGGAATCTGTTGGATTCTATAGTTG | |||||||||||||
| Tm_F_3 | 57.376 | Size_F_3 | 27 | Tm_R_3 | 58.714 | Size_R_3 | 26 | PRODUCT Size | 148 bp | Primer Start | 22467 | Primer End | 22614 |