Primers Available
Organism:Smittium culisetae mitochondrion
Repeat:(A)11
Start:482 End:492
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AGTGGCGAACAAAATTTGAA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AGAGGATGGTTCCCCTTTTT | |||||||||||||
| Tm_F_1 | 58.267 | Size_F_1 | 20 | Tm_R_1 | 58.896 | Size_R_1 | 20 | PRODUCT Size | 155 bp | Primer Start | 455 | Primer End | 609 |
| FORWARD_PRIMER_2 | |||||||||||||
| TGGCGAACAAAATTTGAAAAG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TCTTAGAGGATGGTTCCCCTT | |||||||||||||
| Tm_F_2 | 60.095 | Size_F_2 | 21 | Tm_R_2 | 59.044 | Size_R_2 | 21 | PRODUCT Size | 157 bp | Primer Start | 457 | Primer End | 613 |
| FORWARD_PRIMER_3 | |||||||||||||
| CCGAGATTCAAAAAGTAGTGGC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AGAGGATGGTTCCCCTTTTT | |||||||||||||
| Tm_F_3 | 60.129 | Size_F_3 | 22 | Tm_R_3 | 58.896 | Size_R_3 | 20 | PRODUCT Size | 171 bp | Primer Start | 439 | Primer End | 609 |