Primers Available
Organism: Doliolum nationalis mitochondrion
Repeat:(A)40ctcgttgtcgttattattttcttataagtgcccaatatattctaatcctcccgcgcgtgcgcgcgaatgttaggtaaaccctttg(A)14ttttttttttctaccctaccccctttccgaaaaag(A)19
Start:13349 End:13541
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AGAGAACGTTAAGTAAGCCCCC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CTGCATTTTCGCGCATTTGC | |||||||||||||
| Tm_F_1 | 59.764 | Size_F_1 | 22 | Tm_R_1 | 60.247 | Size_R_1 | 20 | PRODUCT Size | 277 bp | Primer Start | 13324 | Primer End | 13600 |
| FORWARD_PRIMER_2 | |||||||||||||
| AGAGAACGTTAAGTAAGCCCCC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CCTGCATTTTCGCGCATTTG | |||||||||||||
| Tm_F_2 | 59.764 | Size_F_2 | 22 | Tm_R_2 | 59.632 | Size_R_2 | 20 | PRODUCT Size | 278 bp | Primer Start | 13324 | Primer End | 13601 |
| FORWARD_PRIMER_3 | |||||||||||||
| TGTCCCTAGCTTTGCCCAAG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TGTGAAACATAAGCGGCATTCA | |||||||||||||
| Tm_F_3 | 59.962 | Size_F_3 | 20 | Tm_R_3 | 59.183 | Size_R_3 | 22 | PRODUCT Size | 270 bp | Primer Start | 13306 | Primer End | 13575 |