Primers Available
Organism:Kluyveromyces thermotolerans mitochondrion
Repeat:(C)10
Start:18632 End:18641
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TTAATACTCCCTCCCTCCCC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TTCGTGTGTAAAACGAACGC | |||||||||||||
| Tm_F_1 | 59.238 | Size_F_1 | 20 | Tm_R_1 | 59.776 | Size_R_1 | 20 | PRODUCT Size | 278 bp | Primer Start | 18468 | Primer End | 18745 |
| FORWARD_PRIMER_2 | |||||||||||||
| GGGGGTGTTTATAGGGGGAT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TTCGTGTGTAAAACGAACGC | |||||||||||||
| Tm_F_2 | 60.972 | Size_F_2 | 20 | Tm_R_2 | 59.776 | Size_R_2 | 20 | PRODUCT Size | 237 bp | Primer Start | 18509 | Primer End | 18745 |
| FORWARD_PRIMER_3 | |||||||||||||
| GGGGTGTTTATAGGGGGATT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTCGTGTGTAAAACGAACGC | |||||||||||||
| Tm_F_3 | 58.891 | Size_F_3 | 20 | Tm_R_3 | 59.776 | Size_R_3 | 20 | PRODUCT Size | 236 bp | Primer Start | 18510 | Primer End | 18745 |