Primers Available
Organism: Hyla chinensis mitochondrion
Repeat:(C)14attacctcgcacggtttccccacagatcttaatcaaaagtagatcttctctaaacccaatgctt(CCA)4
Start:17447 End:17536
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CGGTTTTTCTATGGGGGCCT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GGAAACCGTGCGAAGTGATG | |||||||||||||
| Tm_F_1 | 60.034 | Size_F_1 | 20 | Tm_R_1 | 59.834 | Size_R_1 | 20 | PRODUCT Size | 245 bp | Primer Start | 17349 | Primer End | 17593 |
| FORWARD_PRIMER_2 | |||||||||||||
| TATGGGGGCCTATCGTAGCT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GGAAACCGTGCGAAGTGATG | |||||||||||||
| Tm_F_2 | 59.588 | Size_F_2 | 20 | Tm_R_2 | 59.834 | Size_R_2 | 20 | PRODUCT Size | 236 bp | Primer Start | 17358 | Primer End | 17593 |
| FORWARD_PRIMER_3 | |||||||||||||
| GGGGCCTATCGTAGCTCTCA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GGAAACCGTGCGAAGTGATG | |||||||||||||
| Tm_F_3 | 60.54 | Size_F_3 | 20 | Tm_R_3 | 59.834 | Size_R_3 | 20 | PRODUCT Size | 232 bp | Primer Start | 17362 | Primer End | 17593 |