Primers Available
Organism: Desmognathus fuscus mitochondrion
Repeat:(TAA)4
Start:4487 End:4498
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCCCCAAACCTCACACTCCT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CCTGATAGTGGGGGAAGTCC | |||||||||||||
| Tm_F_1 | 60.401 | Size_F_1 | 20 | Tm_R_1 | 58.868 | Size_R_1 | 20 | PRODUCT Size | 188 bp | Primer Start | 4425 | Primer End | 4612 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTCCCCAAACCTCACACTCC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CCTGATAGTGGGGGAAGTCC | |||||||||||||
| Tm_F_2 | 59.523 | Size_F_2 | 20 | Tm_R_2 | 58.868 | Size_R_2 | 20 | PRODUCT Size | 189 bp | Primer Start | 4424 | Primer End | 4612 |
| FORWARD_PRIMER_3 | |||||||||||||
| CCCCAAACCTCACACTCCTT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CCTGATAGTGGGGGAAGTCC | |||||||||||||
| Tm_F_3 | 59.521 | Size_F_3 | 20 | Tm_R_3 | 58.868 | Size_R_3 | 20 | PRODUCT Size | 187 bp | Primer Start | 4426 | Primer End | 4612 |