Primers Available
Organism: Desmognathus fuscus mitochondrion
Repeat:(CAA)4
Start:10638 End:10649
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GCAGGCTCACTACCCCTTTT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CCTGCCACTGGAGCTTCTAC | |||||||||||||
| Tm_F_1 | 59.962 | Size_F_1 | 20 | Tm_R_1 | 60.108 | Size_R_1 | 20 | PRODUCT Size | 218 bp | Primer Start | 10543 | Primer End | 10760 |
| FORWARD_PRIMER_2 | |||||||||||||
| TAGCAGGCTCACTACCCCTT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CCTGCCACTGGAGCTTCTAC | |||||||||||||
| Tm_F_2 | 59.957 | Size_F_2 | 20 | Tm_R_2 | 60.108 | Size_R_2 | 20 | PRODUCT Size | 220 bp | Primer Start | 10541 | Primer End | 10760 |
| FORWARD_PRIMER_3 | |||||||||||||
| TTAGCAGGCTCACTACCCCT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CCTGCCACTGGAGCTTCTAC | |||||||||||||
| Tm_F_3 | 59.957 | Size_F_3 | 20 | Tm_R_3 | 60.108 | Size_R_3 | 20 | PRODUCT Size | 221 bp | Primer Start | 10540 | Primer End | 10760 |