Primers Available
Organism: Desmognathus fuscus mitochondrion
Repeat:(ATT)4
Start:4119 End:4130
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AGATACATCACCCCCGGTCA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ACGTCTGGGAGCCATATGTG | |||||||||||||
| Tm_F_1 | 60.032 | Size_F_1 | 20 | Tm_R_1 | 59.534 | Size_R_1 | 20 | PRODUCT Size | 217 bp | Primer Start | 3973 | Primer End | 4189 |
| FORWARD_PRIMER_2 | |||||||||||||
| TGCGTGATACACTGGCGAAT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ACGTCTGGGAGCCATATGTG | |||||||||||||
| Tm_F_2 | 60.108 | Size_F_2 | 20 | Tm_R_2 | 59.534 | Size_R_2 | 20 | PRODUCT Size | 123 bp | Primer Start | 4067 | Primer End | 4189 |
| FORWARD_PRIMER_3 | |||||||||||||
| ATGCGTGATACACTGGCGAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ACGTCTGGGAGCCATATGTG | |||||||||||||
| Tm_F_3 | 60.108 | Size_F_3 | 20 | Tm_R_3 | 59.534 | Size_R_3 | 20 | PRODUCT Size | 124 bp | Primer Start | 4066 | Primer End | 4189 |