Primers Available
Organism: Desmognathus fuscus mitochondrion
Repeat:(AT)7
Start:1757 End:1770
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AGCCACCATAAACTGCGTCA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TGTTCCTGTGTTGGGTTAACA | |||||||||||||
| Tm_F_1 | 59.964 | Size_F_1 | 20 | Tm_R_1 | 57.632 | Size_R_1 | 21 | PRODUCT Size | 248 bp | Primer Start | 1568 | Primer End | 1815 |
| FORWARD_PRIMER_2 | |||||||||||||
| GTTGGCCTAAAAGCAGCCAC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TGTTCCTGTGTTGGGTTAACA | |||||||||||||
| Tm_F_2 | 60.038 | Size_F_2 | 20 | Tm_R_2 | 57.632 | Size_R_2 | 21 | PRODUCT Size | 262 bp | Primer Start | 1554 | Primer End | 1815 |
| FORWARD_PRIMER_3 | |||||||||||||
| CTGTTGGCCTAAAAGCAGCC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TGTTCCTGTGTTGGGTTAACA | |||||||||||||
| Tm_F_3 | 59.755 | Size_F_3 | 20 | Tm_R_3 | 57.632 | Size_R_3 | 21 | PRODUCT Size | 264 bp | Primer Start | 1552 | Primer End | 1815 |