Primers Available
Organism: Desmognathus fuscus mitochondrion
Repeat:(AAT)4
Start:10105 End:10116
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TAGCCACTACCCGAACACAC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ATGGGAGGAGTCAACAGGTT | |||||||||||||
| Tm_F_1 | 59.395 | Size_F_1 | 20 | Tm_R_1 | 58.253 | Size_R_1 | 20 | PRODUCT Size | 278 bp | Primer Start | 10026 | Primer End | 10303 |
| FORWARD_PRIMER_2 | |||||||||||||
| TAGCCACTACCCGAACACAC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AATGGGAGGAGTCAACAGGT | |||||||||||||
| Tm_F_2 | 59.395 | Size_F_2 | 20 | Tm_R_2 | 58.253 | Size_R_2 | 20 | PRODUCT Size | 279 bp | Primer Start | 10026 | Primer End | 10304 |
| FORWARD_PRIMER_3 | |||||||||||||
| GTAGCCACTACCCGAACACA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ATGGGAGGAGTCAACAGGTT | |||||||||||||
| Tm_F_3 | 59.395 | Size_F_3 | 20 | Tm_R_3 | 58.253 | Size_R_3 | 20 | PRODUCT Size | 279 bp | Primer Start | 10025 | Primer End | 10303 |