Primers Available
Organism: Desmognathus wrighti mitochondrion
Repeat:(TTA)4
Start:5803 End:5814
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CCATGCAGGAGCCTCTGTAG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AAACTGGGTCTCCTCCACCT | |||||||||||||
| Tm_F_1 | 59.894 | Size_F_1 | 20 | Tm_R_1 | 60.104 | Size_R_1 | 20 | PRODUCT Size | 278 bp | Primer Start | 5633 | Primer End | 5910 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCATGCAGGAGCCTCTGTAG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ATGCCAGCTGCTAACACTGG | |||||||||||||
| Tm_F_2 | 59.894 | Size_F_2 | 20 | Tm_R_2 | 60.679 | Size_R_2 | 20 | PRODUCT Size | 207 bp | Primer Start | 5633 | Primer End | 5839 |
| FORWARD_PRIMER_3 | |||||||||||||
| CCATGCAGGAGCCTCTGTAG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AAAACTGGGTCTCCTCCACC | |||||||||||||
| Tm_F_3 | 59.894 | Size_F_3 | 20 | Tm_R_3 | 59.228 | Size_R_3 | 20 | PRODUCT Size | 279 bp | Primer Start | 5633 | Primer End | 5911 |