Primers Available
Organism: Desmognathus wrighti mitochondrion
Repeat:(TAAA)4
Start:1497 End:1512
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GCGAAAAACCAAACGAGCCT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GTGGCTGCTTTTAGGCCAAC | |||||||||||||
| Tm_F_1 | 59.97 | Size_F_1 | 20 | Tm_R_1 | 60.038 | Size_R_1 | 20 | PRODUCT Size | 203 bp | Primer Start | 1358 | Primer End | 1560 |
| FORWARD_PRIMER_2 | |||||||||||||
| AGGCGAAAAACCAAACGAGC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GTGGCTGCTTTTAGGCCAAC | |||||||||||||
| Tm_F_2 | 59.97 | Size_F_2 | 20 | Tm_R_2 | 60.038 | Size_R_2 | 20 | PRODUCT Size | 205 bp | Primer Start | 1356 | Primer End | 1560 |
| FORWARD_PRIMER_3 | |||||||||||||
| GCGAAAAACCAAACGAGCCT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TGGCTGCTTTTAGGCCAACA | |||||||||||||
| Tm_F_3 | 59.97 | Size_F_3 | 20 | Tm_R_3 | 60.469 | Size_R_3 | 20 | PRODUCT Size | 202 bp | Primer Start | 1358 | Primer End | 1559 |