Primers Available
Organism: Desmognathus wrighti mitochondrion
Repeat:(TAA)4
Start:4457 End:4468
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CTACTCCTCCATCGCACACC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AATCCGGATGTTGGTGGGAG | |||||||||||||
| Tm_F_1 | 59.897 | Size_F_1 | 20 | Tm_R_1 | 59.744 | Size_R_1 | 20 | PRODUCT Size | 240 bp | Primer Start | 4346 | Primer End | 4585 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCTACTCCTCCATCGCACAC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AATCCGGATGTTGGTGGGAG | |||||||||||||
| Tm_F_2 | 59.897 | Size_F_2 | 20 | Tm_R_2 | 59.744 | Size_R_2 | 20 | PRODUCT Size | 241 bp | Primer Start | 4345 | Primer End | 4585 |
| FORWARD_PRIMER_3 | |||||||||||||
| TAGCCTACTCCTCCATCGCA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AATCCGGATGTTGGTGGGAG | |||||||||||||
| Tm_F_3 | 59.815 | Size_F_3 | 20 | Tm_R_3 | 59.744 | Size_R_3 | 20 | PRODUCT Size | 244 bp | Primer Start | 4342 | Primer End | 4585 |