Primers Available
Organism: Desmognathus wrighti mitochondrion
Repeat:(AAATA)3
Start:1174 End:1188
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CATCACCCACCACCAACAGA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GTAGCTCGTCTGGTTTCGGG | |||||||||||||
| Tm_F_1 | 59.89 | Size_F_1 | 20 | Tm_R_1 | 60.458 | Size_R_1 | 20 | PRODUCT Size | 227 bp | Primer Start | 1064 | Primer End | 1290 |
| FORWARD_PRIMER_2 | |||||||||||||
| CATCACCCACCACCAACAGA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GGTAGCTCGTCTGGTTTCGG | |||||||||||||
| Tm_F_2 | 59.89 | Size_F_2 | 20 | Tm_R_2 | 60.458 | Size_R_2 | 20 | PRODUCT Size | 228 bp | Primer Start | 1064 | Primer End | 1291 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCATCACCCACCACCAACAG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GTAGCTCGTCTGGTTTCGGG | |||||||||||||
| Tm_F_3 | 59.89 | Size_F_3 | 20 | Tm_R_3 | 60.458 | Size_R_3 | 20 | PRODUCT Size | 228 bp | Primer Start | 1063 | Primer End | 1290 |