Primers Available
Organism: Plethodon petraeus mitochondrion
Repeat:(AACC)3
Start:8129 End:8140
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GTCCTCCCTGCTTGCATCTC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AGGATTGGTGTGCCTTCTGG | |||||||||||||
| Tm_F_1 | 60.463 | Size_F_1 | 20 | Tm_R_1 | 59.961 | Size_R_1 | 20 | PRODUCT Size | 204 bp | Primer Start | 7977 | Primer End | 8180 |
| FORWARD_PRIMER_2 | |||||||||||||
| GTCCTCCCTGCTTGCATCTC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AAGCAGGATTGGTGTGCCTT | |||||||||||||
| Tm_F_2 | 60.463 | Size_F_2 | 20 | Tm_R_2 | 60.179 | Size_R_2 | 20 | PRODUCT Size | 208 bp | Primer Start | 7977 | Primer End | 8184 |
| FORWARD_PRIMER_3 | |||||||||||||
| AAATGGTCCTCCCTGCTTGC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AGGATTGGTGTGCCTTCTGG | |||||||||||||
| Tm_F_3 | 60.613 | Size_F_3 | 20 | Tm_R_3 | 59.961 | Size_R_3 | 20 | PRODUCT Size | 209 bp | Primer Start | 7972 | Primer End | 8180 |