Primers Available
Organism: Rhyacotriton variegatus mitochondrion
Repeat:(TTA)4
Start:8452 End:8463
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CCAACCTAACTGCCGGACAT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CGTGTGCTTGGTGTGTCATT | |||||||||||||
| Tm_F_1 | 60.035 | Size_F_1 | 20 | Tm_R_1 | 59.337 | Size_R_1 | 20 | PRODUCT Size | 215 bp | Primer Start | 8350 | Primer End | 8564 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCAACCTAACTGCCGGACAT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TATGGAAGGCGTGTGCTTGG | |||||||||||||
| Tm_F_2 | 60.035 | Size_F_2 | 20 | Tm_R_2 | 60.676 | Size_R_2 | 20 | PRODUCT Size | 224 bp | Primer Start | 8350 | Primer End | 8573 |
| FORWARD_PRIMER_3 | |||||||||||||
| TAACCGCCAACCTAACTGCC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CGTGTGCTTGGTGTGTCATT | |||||||||||||
| Tm_F_3 | 60.322 | Size_F_3 | 20 | Tm_R_3 | 59.337 | Size_R_3 | 20 | PRODUCT Size | 221 bp | Primer Start | 8344 | Primer End | 8564 |