Primers Available
Organism: Rhyacotriton variegatus mitochondrion
Repeat:(TCCTC)3
Start:3676 End:3690
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TGAACACGAGCCTCATACCC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AGTGGTGTAATGGGTGCACG | |||||||||||||
| Tm_F_1 | 59.465 | Size_F_1 | 20 | Tm_R_1 | 60.605 | Size_R_1 | 20 | PRODUCT Size | 278 bp | Primer Start | 3477 | Primer End | 3754 |
| FORWARD_PRIMER_2 | |||||||||||||
| AACACGAGCCTCATACCCTC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AGTGGTGTAATGGGTGCACG | |||||||||||||
| Tm_F_2 | 59.175 | Size_F_2 | 20 | Tm_R_2 | 60.605 | Size_R_2 | 20 | PRODUCT Size | 276 bp | Primer Start | 3479 | Primer End | 3754 |
| FORWARD_PRIMER_3 | |||||||||||||
| GAACACGAGCCTCATACCCT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AGTGGTGTAATGGGTGCACG | |||||||||||||
| Tm_F_3 | 59.175 | Size_F_3 | 20 | Tm_R_3 | 60.605 | Size_R_3 | 20 | PRODUCT Size | 277 bp | Primer Start | 3478 | Primer End | 3754 |