Primers Available
Organism: Rhyacotriton variegatus mitochondrion
Repeat:(G)14
Start:15482 End:15495
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCCAGGCTCTTCCATTCCTT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GGGTAGGGTTGTCTATCTACAGG | |||||||||||||
| Tm_F_1 | 58.326 | Size_F_1 | 20 | Tm_R_1 | 59.104 | Size_R_1 | 23 | PRODUCT Size | 257 bp | Primer Start | 15265 | Primer End | 15521 |
| FORWARD_PRIMER_2 | |||||||||||||
| ATCCAGGCTCTTCCATTCCT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GGGTAGGGTTGTCTATCTACAGG | |||||||||||||
| Tm_F_2 | 58.096 | Size_F_2 | 20 | Tm_R_2 | 59.104 | Size_R_2 | 23 | PRODUCT Size | 258 bp | Primer Start | 15264 | Primer End | 15521 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCCAGGCTCTTCCATTCCTT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AGGGTAGGGTTGTCTATCTACAGG | |||||||||||||
| Tm_F_3 | 58.326 | Size_F_3 | 20 | Tm_R_3 | 60.388 | Size_R_3 | 24 | PRODUCT Size | 258 bp | Primer Start | 15265 | Primer End | 15522 |