Primers Available
Organism: Rhyacotriton variegatus mitochondrion
Repeat:(CAA)4
Start:13591 End:13602
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AACAGCCCGTAATGAACCAT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GGTTGACGTGTTGGAACAGG | |||||||||||||
| Tm_F_1 | 57.493 | Size_F_1 | 20 | Tm_R_1 | 59.339 | Size_R_1 | 20 | PRODUCT Size | 201 bp | Primer Start | 13456 | Primer End | 13656 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCTAACAGCCCGTAATGAACCA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GGTTGACGTGTTGGAACAGG | |||||||||||||
| Tm_F_2 | 59.432 | Size_F_2 | 22 | Tm_R_2 | 59.339 | Size_R_2 | 20 | PRODUCT Size | 204 bp | Primer Start | 13453 | Primer End | 13656 |
| FORWARD_PRIMER_3 | |||||||||||||
| CTAACAGCCCGTAATGAACCA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GGTTGACGTGTTGGAACAGG | |||||||||||||
| Tm_F_3 | 58.019 | Size_F_3 | 21 | Tm_R_3 | 59.339 | Size_R_3 | 20 | PRODUCT Size | 203 bp | Primer Start | 13454 | Primer End | 13656 |