Primers Available
Organism: Rhyacotriton variegatus mitochondrion
Repeat:(AAAT)4
Start:366 End:381
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GCCGCGGTTATACGAAAGAC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ACCGCCAAGTCCTTTGAGTT | |||||||||||||
| Tm_F_1 | 59.426 | Size_F_1 | 20 | Tm_R_1 | 59.817 | Size_R_1 | 20 | PRODUCT Size | 266 bp | Primer Start | 309 | Primer End | 574 |
| FORWARD_PRIMER_2 | |||||||||||||
| CGCCGCGGTTATACGAAAGA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ACCGCCAAGTCCTTTGAGTT | |||||||||||||
| Tm_F_2 | 60.867 | Size_F_2 | 20 | Tm_R_2 | 59.817 | Size_R_2 | 20 | PRODUCT Size | 267 bp | Primer Start | 308 | Primer End | 574 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCGGTCAATATCGTGCCAGC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TCGTAGTACTCTGGCGGAGA | |||||||||||||
| Tm_F_3 | 60.53 | Size_F_3 | 20 | Tm_R_3 | 59.461 | Size_R_3 | 20 | PRODUCT Size | 256 bp | Primer Start | 288 | Primer End | 543 |