Primers Available
Organism: Aneides flavipunctatus mitochondrion
Repeat:(TTA)4
Start:10428 End:10439
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TTGGTTCTCACCTGCTGACT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GGCGTTCTGTTTGGTTTCCTC | |||||||||||||
| Tm_F_1 | 58.868 | Size_F_1 | 20 | Tm_R_1 | 60.002 | Size_R_1 | 21 | PRODUCT Size | 232 bp | Primer Start | 10234 | Primer End | 10465 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTGGTTCTCACCTGCTGACT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TCCTGCATTTAGGCGTTCTGT | |||||||||||||
| Tm_F_2 | 58.868 | Size_F_2 | 20 | Tm_R_2 | 59.997 | Size_R_2 | 21 | PRODUCT Size | 243 bp | Primer Start | 10234 | Primer End | 10476 |
| FORWARD_PRIMER_3 | |||||||||||||
| AGCATTTTCCGCAACAGAACT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AGGGGTAGTGATCCGGCTAG | |||||||||||||
| Tm_F_3 | 59.044 | Size_F_3 | 21 | Tm_R_3 | 60.179 | Size_R_3 | 20 | PRODUCT Size | 156 bp | Primer Start | 10362 | Primer End | 10517 |