Primers Available
Organism: Aneides flavipunctatus mitochondrion
Repeat:(AAT)5(AATA)3*
Start:10180 End:10203
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CACCCATGGATCAGACCACC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AGTCAGCAGGTGAGAACCAA | |||||||||||||
| Tm_F_1 | 60.107 | Size_F_1 | 20 | Tm_R_1 | 58.868 | Size_R_1 | 20 | PRODUCT Size | 257 bp | Primer Start | 9997 | Primer End | 10253 |
| FORWARD_PRIMER_2 | |||||||||||||
| ACCCATGGATCAGACCACCT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AGTCAGCAGGTGAGAACCAA | |||||||||||||
| Tm_F_2 | 60.253 | Size_F_2 | 20 | Tm_R_2 | 58.868 | Size_R_2 | 20 | PRODUCT Size | 256 bp | Primer Start | 9998 | Primer End | 10253 |
| FORWARD_PRIMER_3 | |||||||||||||
| AATGGTTGCAACTACCCGCA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AGTCAGCAGGTGAGAACCAA | |||||||||||||
| Tm_F_3 | 60.539 | Size_F_3 | 20 | Tm_R_3 | 58.868 | Size_R_3 | 20 | PRODUCT Size | 275 bp | Primer Start | 9979 | Primer End | 10253 |