Primers Available
Organism: Aneides flavipunctatus mitochondrion
Repeat:(AAT)5
Start:4514 End:4528
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CCTCTATTGCACACCTGGGT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GGAGGTTGGTGGGAGTCCC | |||||||||||||
| Tm_F_1 | 59.379 | Size_F_1 | 20 | Tm_R_1 | 61.604 | Size_R_1 | 19 | PRODUCT Size | 230 bp | Primer Start | 4332 | Primer End | 4561 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCTCTATTGCACACCTGGGT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CGGAGGTTGGTGGGAGTC | |||||||||||||
| Tm_F_2 | 59.379 | Size_F_2 | 20 | Tm_R_2 | 59.337 | Size_R_2 | 18 | PRODUCT Size | 231 bp | Primer Start | 4332 | Primer End | 4562 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCCTCTATTGCACACCTGGG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GGAGGTTGGTGGGAGTCCC | |||||||||||||
| Tm_F_3 | 59.088 | Size_F_3 | 20 | Tm_R_3 | 61.604 | Size_R_3 | 19 | PRODUCT Size | 231 bp | Primer Start | 4331 | Primer End | 4561 |