Primers Available
Organism: Saccharum hybrid cultivar NCo 310 chloroplast
Repeat:(T)13
Start:81244 End:81256
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AACCCGGCTGCTCTTTATTT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ACCTGATTCTAGGCTGTGAAGC | |||||||||||||
| Tm_F_1 | 57.784 | Size_F_1 | 20 | Tm_R_1 | 60.093 | Size_R_1 | 22 | PRODUCT Size | 251 bp | Primer Start | 81171 | Primer End | 81421 |
| FORWARD_PRIMER_2 | |||||||||||||
| AACCCGGCTGCTCTTTATTT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CCTGATTCTAGGCTGTGAAGCA | |||||||||||||
| Tm_F_2 | 57.784 | Size_F_2 | 20 | Tm_R_2 | 60.093 | Size_R_2 | 22 | PRODUCT Size | 250 bp | Primer Start | 81171 | Primer End | 81420 |
| FORWARD_PRIMER_3 | |||||||||||||
| GAATCCTGCAATGGAGCTTCC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AGGGATCTGGATAAAAGGAGGGA | |||||||||||||
| Tm_F_3 | 59.32 | Size_F_3 | 21 | Tm_R_3 | 60.05 | Size_R_3 | 23 | PRODUCT Size | 280 bp | Primer Start | 81112 | Primer End | 81391 |